For Sale Nikon Eclipse TI-E PFS Inverted Fluorescence Motorized DIC Microscope with Motorized Stage
Pre-owned microscope (this microscope can be fully customized)
• Fully Functional
• Serviced & in Stock
• Good cosmetic condition
• Comes with Brand New Fluorescence Filter Sets
• Continental US Warranty 120 Days & 60 Days International.
• An extended warranty option and training package are available.
The Predecessor to Nikon Ti2-E PFS Inverted Fluorescence Microscope
This is what you will receive before customizing this microscope:
Standard Nikon Eclipse TI-E PFS Inverted Microscope Configuration:
Microscopy Techniques:
Brightfield with DIC and Fluorescence Microscopy with PFS (Perfect Focus automated drift control)
Microscope Base:
• Nikon Eclipse TI-E Base
• with a Motorized(Automated) Z-Axis Drive
• TI-HUBC/A Controller
Microscope Head:
• Binocular Head
• Two Eyepieces
Fluorescence Light Path:
• TI-FL Fluorescence Light Path
Fluorescence Illumination:
• Lumencor Sola LED Light Source
• Collimating adapter
• Light guide
Fluorescence filter cube turret:
• TI-FLC-E 6-position Motorized Turret
Fluorescence filters:
• Three empty filter cubes
• Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
Brightfield Light Path:
• Illumination Tower
Brightfield Illumination:
• D-LH/LC 12V 100W Halogen Lamp
• TE-PS100 power supply
Objective Turret:
• TI-ND6-PFS 6-Position Motorized Nosepiece
• Nikon Perfect Focus System (PFS)
Objectives:
• 4x/0.13 Plan Fluor
• 10x/0.30 Plan Fluor
• 20x/0.75 CFI Plan Apo VC
Condenser:
• TI-C Mechanical Condenser w/ LWD 0.52
DIC Components:
• Polarizer
• DIC M Condenser Prism
• DIC M Objective Prism
• Analyzer
Stage:
• Motorized XY Stage
• LEP MAC6000 Stage Controller
• Joystick
Camera ports:
• 100% Eyepiece, 0% Left Camera
• 20% Eyepiece, 80% Left Camera
• 0% Eyepiece, 100% Left Camera
Camera adapter:
• 1x C-mount adapter
Camera:
• CMOS USB 3.0 10MP Camera
Computer & Software:
• Dell laptop computer with Windows 10
• Micromanager w/ImageJ analysis software
• Camera software
Accessories:
• Necessary power and communication cables
This configuration includes any 3 of these new filter sets.
• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)
Automated/motorized capabilities of this microscope:
Installed automation/motorized components:
• Motorized lightpath
• Motorized/automated Z-Axis Drive
• Motorized filter cube turret
• Motorized objective(nosepiece) turret
• Motorized drift focus (PFS) control
• Motorized XY Stage
Benefits of motorized/Automated Z-Axis drive:
• Automatic parfocality adjustment
• The ability to recall previously saved focus levels
• Automated management of Z positioning/ Fine-coarse control
How the Nikon Perfect Focus System Works
• Infrared Light Source: Uses IR light, reducing photodamage and bleaching in live cells.
• Dedicated Optical Path: IR light hits the sample via a path that includes a dichroic mirror, allowing visible light through.
• Reflected Light Detection: IR light reflection from the cover glass-sample surface is detected.
• Focus Offset Detection: Continuous measurement of reflected IR light position to detect focal shifts or drifts.
• Automatic Correction: Corrects focus in real-time by adjusting the lens or stage when shifts or drifts are detected.
Benefits of Nikon Perfect Focus System(PFS):
• Near Effortless swapping of samples at different focal ranges: Swapping samples with focal planes within the PFS range allows for fast refocusing.
• Long-term Observations: Enables the continuous observation of live cells over extended periods.
• Minimizing Phototoxicity: By keeping the focus precise, the need for frequent manual adjustments and the associated increased light exposure is reduced.
• Automated Multi-well Plate Scanning: Allows for the automated and consistent focus across different wells in a multi-well plate.
• Embryo Imaging: Provides the stability needed to image developing embryos over time.
• Neuron Activity Studies: The system can maintain focus on specific areas of the brain or neural cultures over long periods.
• Reduced Photobleaching: By maintaining focus with minimal adjustments, the PFS helps in reducing exposure to intense light.
• Improved Z-stacking: Ensures consistent focus across multiple z-planes.
• Stable Time-lapse Sequences: Vital for capturing time-lapse sequences where a consistent focus is required to document changes over time accurately.
• Surface Topography Studies: Maintaining focus is essential for accurately mapping surface topographies and understanding material properties.
• Drug Response Monitoring: Allows for the detailed observation of cellular responses to drug treatments over time.
This microscope's imaging capabilities:
Imaging software highlights:
• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others
• Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
• Multi-frame (Time lapse/Image acquisition over time)
• Multi-slice (Z coordinate/Z Stacking)
• Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage
We can customize this microscope to fit your requirements:
Add Microscopy Techniques (In addition to the installed Brightfield with DIC and Fluorescence)
• Phase Contrast or Polarization Add-on Components may be added (subject to availability)
Increase Maximum Magnification and Image Quality:
• Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
• We can add standard higher magnification Nikon microscope objectives
• We can add high-end NikonApochromat objectives
Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.
• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources
We will also ensure the fluorescence light source matches your selected filter sets.
Enhance imaging capabilities:
• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.
Add other Motorized/Automation Capabilities:
In addition to installed motorized capabilities, the microscope can be configured
• Motorized Condenser or Motorized high-speed shutter
We can configure it to work with the sample medium you need:
• Slide, Petri dish, microplate, etc
Commonly Requested Customer Upgrades
• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
We are excited to help build and configure the right microscope for you!
Nikon Eclipse TI-E PFS Fluorescence Microscope Overview
(Final microscope capabilities will depend on the modifications you request)
Suited for the following applications as configured:
• Time-lapse photography: Captures sequential images to monitor changes over time.
• Fluorescence microscopy: Ideal for fluorescent labeling and imaging of cells and tissues.
• Material science research: Suitable for examining material properties and structures.
• Biological research: Essential for cellular structure, function, and behavior studies.
• DIC: Enhances visibility of transparent specimens without staining.
• Pharmacological studies: Facilitates the investigation of drug effects on cells and tissues.
• Multi-dimensional imaging: Allows for the acquisition of images in multiple planes and dimensions.
• Biotechnology applications: Enables genetic engineering and molecular biology research.
• Educational purposes: Serves as a teaching tool in universities and research institutes.
Other potential applications:
• Quantitative analysis: Supports measurements and quantification of specimen features.
• Phase contrast: Enhances visibility of transparent specimens without staining.
• High-resolution imaging: Provides clear, detailed images for in-depth analysis of samples.
• Live cell observation: Enables detailed study of live cell dynamics in real-time.
Why should you buy from us?
Our Advantage
• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)
Our Guarantee
• It will work!
• Continental US Warranty 120 Days & 60 Days International.
• An extended warranty may be available.
• You get after-sales technical support.
• Smooth process from the initial quote to order fulfillment.
• Customer references are available.
*SM* Small Crate
200#
PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE
Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.
Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application
Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.
Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.
Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.
Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope
Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives.
Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.
Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.
Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.
Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.
Q: Have you packed and shipped microscopes in the past?
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.
Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.
Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.
DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.
ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.
Return Policy
Items are sold as-is with no returns or refunds available unless explicitly stated.
