The Coulter AcT 5-part differential (5 diff) autoloader (AL) hematology analyzer from Beckman
Coulter (Fullerton, CA, USA) was evaluated at the Florida Medical Center in Fort Lauderdale,
Florida, an acute care hospital facility. The AcT 5 diff AL is a new, fully automated bench-top 5-
part differential hematology analyzer with automatic loading and sampling. It is designed as a
front-line instrument for small- to medium-sized laboratories or as a backup in larger
laboratories. We evaluated the performance of the AcT 5 diff AL for complete blood counts
(CBCs). The Coulter Onyx AL was used as the reference instrument and the 5-part differential
against the reference 400-cell manual differential count (2 operators x 200 cells). A total of 140
patient samples were analyzed in the study, with most of the samples obtained from our own
laboratory workload.
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